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STEMCELL Technologies Inc easysep rat cd4+t/cd45r+b cell isolation kit
Easysep Rat Cd4+T/Cd45r+B Cell Isolation Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/easysep+rat+cd4+++t+cell+isolation+kit/easysep+human+t+cell+isolation+kit/pm37995933-87-8-16
Average 90 stars, based on 1 article reviews
easysep rat cd4+t/cd45r+b cell isolation kit - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

In Vitro:

Article Title: Identification of essential modules regulating T cell migration to the central nervous system in multiple sclerosis
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySepTM Rat CD4 + T Cell Isolation Kit (StemCell technologies, 19642), before purification by sorting.

Article Title: A Dominant Role for Regulatory T Cells in Protecting Females Against Pulmonary Hypertension
Article Snippet: Negative selection was performed using EasySep TM Rat CD4 + T Cell Isolation Kit (#19642, STEMCELL Technologies) followed by labeling CD4 + T cells with anti-CD25-PE antibody; the CD25 high cells subset was selected with the EasySep TM PE Selection Kit (#18557, STEMCELL Technologies).

Article Title: Cardiac injection of USSC boosts remuscularization of the infarcted heart by shaping the T-cell response.
Article Snippet: Coculture of USSC with CD4+ T cells Rat CD4+ T cells were magnetically sorted by EasySep Rat CD4+ T Cell Isolation Kit (StemCell).

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model.
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4+ T cells were enriched using the EasySep Rat CD4+ T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Mesenchymal stem cells exert renoprotection via extracellular vesicle-mediated modulation of M2 macrophages and spleen-kidney network
Article Snippet: Magnetic cell separation was performed to harvest CD4-positive cell-rich samples using an EasySep rat CD4 + T-cell isolation kit (STEMCELL Technologies, Vancouver, BC, Canada).

Article Title: Leflunomide ameliorates experimental autoimmune myasthenia gravis by regulating humoral and cellular immune responses.
Article Snippet: Leflunomide, an immunosuppressive disease-modifying anti-rheumatic drug (DMARD), is widely used in the treatment of rheumatoid arthritis (RA), psoriatic arthritis (PA) as well as multiple sclerosis.. However, its role in myasthenia gravis (MG) has not yet been clearly explored.. Here, we investigated the effect of leflunomide on experimental autoimmune myasthenia gravis (EAMG) in vivo and in vitro.

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySep Rat CD4 + T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Targeting HIF-1α alleviates the inflammatory responses and rebuilds the CD4 + T cell subsets balance in the experimental autoimmune myasthenia gravis inflammation model via regulating cellular and humoral immunity.
Article Snippet: Background: Cells and tissues in an inflammatory state are usually hypoxic.. The hypoxic environment can affect the differentiation of immune cells and produce Hypoxia-inducible Factor-1α (HIF-1α).. Inflammation is also a major contributor to the development and deterioration of Myasthenia Gravis (MG).

Purification:

Article Title: Identification of essential modules regulating T cell migration to the central nervous system in multiple sclerosis
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySepTM Rat CD4 + T Cell Isolation Kit (StemCell technologies, 19642), before purification by sorting.

Article Title: A Dominant Role for Regulatory T Cells in Protecting Females Against Pulmonary Hypertension
Article Snippet: Negative selection was performed using EasySep TM Rat CD4 + T Cell Isolation Kit (#19642, STEMCELL Technologies) followed by labeling CD4 + T cells with anti-CD25-PE antibody; the CD25 high cells subset was selected with the EasySep TM PE Selection Kit (#18557, STEMCELL Technologies).

Article Title: Cardiac injection of USSC boosts remuscularization of the infarcted heart by shaping the T-cell response.
Article Snippet: Coculture of USSC with CD4+ T cells Rat CD4+ T cells were magnetically sorted by EasySep Rat CD4+ T Cell Isolation Kit (StemCell).

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model.
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4+ T cells were enriched using the EasySep Rat CD4+ T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Mesenchymal stem cells exert renoprotection via extracellular vesicle-mediated modulation of M2 macrophages and spleen-kidney network
Article Snippet: Magnetic cell separation was performed to harvest CD4-positive cell-rich samples using an EasySep rat CD4 + T-cell isolation kit (STEMCELL Technologies, Vancouver, BC, Canada).

Article Title: Leflunomide ameliorates experimental autoimmune myasthenia gravis by regulating humoral and cellular immune responses.
Article Snippet: Leflunomide, an immunosuppressive disease-modifying anti-rheumatic drug (DMARD), is widely used in the treatment of rheumatoid arthritis (RA), psoriatic arthritis (PA) as well as multiple sclerosis.. However, its role in myasthenia gravis (MG) has not yet been clearly explored.. Here, we investigated the effect of leflunomide on experimental autoimmune myasthenia gravis (EAMG) in vivo and in vitro.

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySep Rat CD4 + T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Targeting HIF-1α alleviates the inflammatory responses and rebuilds the CD4 + T cell subsets balance in the experimental autoimmune myasthenia gravis inflammation model via regulating cellular and humoral immunity.
Article Snippet: Background: Cells and tissues in an inflammatory state are usually hypoxic.. The hypoxic environment can affect the differentiation of immune cells and produce Hypoxia-inducible Factor-1α (HIF-1α).. Inflammation is also a major contributor to the development and deterioration of Myasthenia Gravis (MG).

Cell Isolation:

Article Title: Identification of essential modules regulating T cell migration to the central nervous system in multiple sclerosis
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySepTM Rat CD4 + T Cell Isolation Kit (StemCell technologies, 19642), before purification by sorting.

Article Title: A Dominant Role for Regulatory T Cells in Protecting Females Against Pulmonary Hypertension
Article Snippet: Negative selection was performed using EasySep TM Rat CD4 + T Cell Isolation Kit (#19642, STEMCELL Technologies) followed by labeling CD4 + T cells with anti-CD25-PE antibody; the CD25 high cells subset was selected with the EasySep TM PE Selection Kit (#18557, STEMCELL Technologies).

Article Title: Cardiac injection of USSC boosts remuscularization of the infarcted heart by shaping the T-cell response.
Article Snippet: Coculture of USSC with CD4+ T cells Rat CD4+ T cells were magnetically sorted by EasySep Rat CD4+ T Cell Isolation Kit (StemCell).

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model.
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4+ T cells were enriched using the EasySep Rat CD4+ T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Mesenchymal stem cells exert renoprotection via extracellular vesicle-mediated modulation of M2 macrophages and spleen-kidney network
Article Snippet: Magnetic cell separation was performed to harvest CD4-positive cell-rich samples using an EasySep rat CD4 + T-cell isolation kit (STEMCELL Technologies, Vancouver, BC, Canada).

Article Title: Leflunomide ameliorates experimental autoimmune myasthenia gravis by regulating humoral and cellular immune responses.
Article Snippet: Leflunomide, an immunosuppressive disease-modifying anti-rheumatic drug (DMARD), is widely used in the treatment of rheumatoid arthritis (RA), psoriatic arthritis (PA) as well as multiple sclerosis.. However, its role in myasthenia gravis (MG) has not yet been clearly explored.. Here, we investigated the effect of leflunomide on experimental autoimmune myasthenia gravis (EAMG) in vivo and in vitro.

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySep Rat CD4 + T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Targeting HIF-1α alleviates the inflammatory responses and rebuilds the CD4 + T cell subsets balance in the experimental autoimmune myasthenia gravis inflammation model via regulating cellular and humoral immunity.
Article Snippet: Background: Cells and tissues in an inflammatory state are usually hypoxic.. The hypoxic environment can affect the differentiation of immune cells and produce Hypoxia-inducible Factor-1α (HIF-1α).. Inflammation is also a major contributor to the development and deterioration of Myasthenia Gravis (MG).

Selection:

Article Title: Identification of essential modules regulating T cell migration to the central nervous system in multiple sclerosis
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySepTM Rat CD4 + T Cell Isolation Kit (StemCell technologies, 19642), before purification by sorting.

Article Title: A Dominant Role for Regulatory T Cells in Protecting Females Against Pulmonary Hypertension
Article Snippet: Negative selection was performed using EasySep TM Rat CD4 + T Cell Isolation Kit (#19642, STEMCELL Technologies) followed by labeling CD4 + T cells with anti-CD25-PE antibody; the CD25 high cells subset was selected with the EasySep TM PE Selection Kit (#18557, STEMCELL Technologies).

Article Title: Cardiac injection of USSC boosts remuscularization of the infarcted heart by shaping the T-cell response.
Article Snippet: Coculture of USSC with CD4+ T cells Rat CD4+ T cells were magnetically sorted by EasySep Rat CD4+ T Cell Isolation Kit (StemCell).

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model.
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4+ T cells were enriched using the EasySep Rat CD4+ T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Mesenchymal stem cells exert renoprotection via extracellular vesicle-mediated modulation of M2 macrophages and spleen-kidney network
Article Snippet: Magnetic cell separation was performed to harvest CD4-positive cell-rich samples using an EasySep rat CD4 + T-cell isolation kit (STEMCELL Technologies, Vancouver, BC, Canada).

Article Title: Leflunomide ameliorates experimental autoimmune myasthenia gravis by regulating humoral and cellular immune responses.
Article Snippet: Leflunomide, an immunosuppressive disease-modifying anti-rheumatic drug (DMARD), is widely used in the treatment of rheumatoid arthritis (RA), psoriatic arthritis (PA) as well as multiple sclerosis.. However, its role in myasthenia gravis (MG) has not yet been clearly explored.. Here, we investigated the effect of leflunomide on experimental autoimmune myasthenia gravis (EAMG) in vivo and in vitro.

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySep Rat CD4 + T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Targeting HIF-1α alleviates the inflammatory responses and rebuilds the CD4 + T cell subsets balance in the experimental autoimmune myasthenia gravis inflammation model via regulating cellular and humoral immunity.
Article Snippet: Background: Cells and tissues in an inflammatory state are usually hypoxic.. The hypoxic environment can affect the differentiation of immune cells and produce Hypoxia-inducible Factor-1α (HIF-1α).. Inflammation is also a major contributor to the development and deterioration of Myasthenia Gravis (MG).

Magnetic Cell Separation:

Article Title: Identification of essential modules regulating T cell migration to the central nervous system in multiple sclerosis
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySepTM Rat CD4 + T Cell Isolation Kit (StemCell technologies, 19642), before purification by sorting.

Article Title: A Dominant Role for Regulatory T Cells in Protecting Females Against Pulmonary Hypertension
Article Snippet: Negative selection was performed using EasySep TM Rat CD4 + T Cell Isolation Kit (#19642, STEMCELL Technologies) followed by labeling CD4 + T cells with anti-CD25-PE antibody; the CD25 high cells subset was selected with the EasySep TM PE Selection Kit (#18557, STEMCELL Technologies).

Article Title: Cardiac injection of USSC boosts remuscularization of the infarcted heart by shaping the T-cell response.
Article Snippet: Coculture of USSC with CD4+ T cells Rat CD4+ T cells were magnetically sorted by EasySep Rat CD4+ T Cell Isolation Kit (StemCell).

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model.
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4+ T cells were enriched using the EasySep Rat CD4+ T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Mesenchymal stem cells exert renoprotection via extracellular vesicle-mediated modulation of M2 macrophages and spleen-kidney network
Article Snippet: Magnetic cell separation was performed to harvest CD4-positive cell-rich samples using an EasySep rat CD4 + T-cell isolation kit (STEMCELL Technologies, Vancouver, BC, Canada).

Article Title: Leflunomide ameliorates experimental autoimmune myasthenia gravis by regulating humoral and cellular immune responses.
Article Snippet: Leflunomide, an immunosuppressive disease-modifying anti-rheumatic drug (DMARD), is widely used in the treatment of rheumatoid arthritis (RA), psoriatic arthritis (PA) as well as multiple sclerosis.. However, its role in myasthenia gravis (MG) has not yet been clearly explored.. Here, we investigated the effect of leflunomide on experimental autoimmune myasthenia gravis (EAMG) in vivo and in vitro.

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySep Rat CD4 + T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Targeting HIF-1α alleviates the inflammatory responses and rebuilds the CD4 + T cell subsets balance in the experimental autoimmune myasthenia gravis inflammation model via regulating cellular and humoral immunity.
Article Snippet: Background: Cells and tissues in an inflammatory state are usually hypoxic.. The hypoxic environment can affect the differentiation of immune cells and produce Hypoxia-inducible Factor-1α (HIF-1α).. Inflammation is also a major contributor to the development and deterioration of Myasthenia Gravis (MG).

Isolation:

Article Title: Identification of essential modules regulating T cell migration to the central nervous system in multiple sclerosis
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySepTM Rat CD4 + T Cell Isolation Kit (StemCell technologies, 19642), before purification by sorting.

Article Title: A Dominant Role for Regulatory T Cells in Protecting Females Against Pulmonary Hypertension
Article Snippet: Negative selection was performed using EasySep TM Rat CD4 + T Cell Isolation Kit (#19642, STEMCELL Technologies) followed by labeling CD4 + T cells with anti-CD25-PE antibody; the CD25 high cells subset was selected with the EasySep TM PE Selection Kit (#18557, STEMCELL Technologies).

Article Title: Cardiac injection of USSC boosts remuscularization of the infarcted heart by shaping the T-cell response.
Article Snippet: Coculture of USSC with CD4+ T cells Rat CD4+ T cells were magnetically sorted by EasySep Rat CD4+ T Cell Isolation Kit (StemCell).

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model.
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4+ T cells were enriched using the EasySep Rat CD4+ T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Mesenchymal stem cells exert renoprotection via extracellular vesicle-mediated modulation of M2 macrophages and spleen-kidney network
Article Snippet: Magnetic cell separation was performed to harvest CD4-positive cell-rich samples using an EasySep rat CD4 + T-cell isolation kit (STEMCELL Technologies, Vancouver, BC, Canada).

Article Title: Leflunomide ameliorates experimental autoimmune myasthenia gravis by regulating humoral and cellular immune responses.
Article Snippet: Leflunomide, an immunosuppressive disease-modifying anti-rheumatic drug (DMARD), is widely used in the treatment of rheumatoid arthritis (RA), psoriatic arthritis (PA) as well as multiple sclerosis.. However, its role in myasthenia gravis (MG) has not yet been clearly explored.. Here, we investigated the effect of leflunomide on experimental autoimmune myasthenia gravis (EAMG) in vivo and in vitro.

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySep Rat CD4 + T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Targeting HIF-1α alleviates the inflammatory responses and rebuilds the CD4 + T cell subsets balance in the experimental autoimmune myasthenia gravis inflammation model via regulating cellular and humoral immunity.
Article Snippet: Background: Cells and tissues in an inflammatory state are usually hypoxic.. The hypoxic environment can affect the differentiation of immune cells and produce Hypoxia-inducible Factor-1α (HIF-1α).. Inflammation is also a major contributor to the development and deterioration of Myasthenia Gravis (MG).

Flow Cytometry:

Article Title: Identification of essential modules regulating T cell migration to the central nervous system in multiple sclerosis
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySepTM Rat CD4 + T Cell Isolation Kit (StemCell technologies, 19642), before purification by sorting.

Article Title: A Dominant Role for Regulatory T Cells in Protecting Females Against Pulmonary Hypertension
Article Snippet: Negative selection was performed using EasySep TM Rat CD4 + T Cell Isolation Kit (#19642, STEMCELL Technologies) followed by labeling CD4 + T cells with anti-CD25-PE antibody; the CD25 high cells subset was selected with the EasySep TM PE Selection Kit (#18557, STEMCELL Technologies).

Article Title: Cardiac injection of USSC boosts remuscularization of the infarcted heart by shaping the T-cell response.
Article Snippet: Coculture of USSC with CD4+ T cells Rat CD4+ T cells were magnetically sorted by EasySep Rat CD4+ T Cell Isolation Kit (StemCell).

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model.
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4+ T cells were enriched using the EasySep Rat CD4+ T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Mesenchymal stem cells exert renoprotection via extracellular vesicle-mediated modulation of M2 macrophages and spleen-kidney network
Article Snippet: Magnetic cell separation was performed to harvest CD4-positive cell-rich samples using an EasySep rat CD4 + T-cell isolation kit (STEMCELL Technologies, Vancouver, BC, Canada).

Article Title: Leflunomide ameliorates experimental autoimmune myasthenia gravis by regulating humoral and cellular immune responses.
Article Snippet: Leflunomide, an immunosuppressive disease-modifying anti-rheumatic drug (DMARD), is widely used in the treatment of rheumatoid arthritis (RA), psoriatic arthritis (PA) as well as multiple sclerosis.. However, its role in myasthenia gravis (MG) has not yet been clearly explored.. Here, we investigated the effect of leflunomide on experimental autoimmune myasthenia gravis (EAMG) in vivo and in vitro.

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySep Rat CD4 + T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Targeting HIF-1α alleviates the inflammatory responses and rebuilds the CD4 + T cell subsets balance in the experimental autoimmune myasthenia gravis inflammation model via regulating cellular and humoral immunity.
Article Snippet: Background: Cells and tissues in an inflammatory state are usually hypoxic.. The hypoxic environment can affect the differentiation of immune cells and produce Hypoxia-inducible Factor-1α (HIF-1α).. Inflammation is also a major contributor to the development and deterioration of Myasthenia Gravis (MG).

Co-Culture Assay:

Article Title: Identification of essential modules regulating T cell migration to the central nervous system in multiple sclerosis
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySepTM Rat CD4 + T Cell Isolation Kit (StemCell technologies, 19642), before purification by sorting.

Article Title: A Dominant Role for Regulatory T Cells in Protecting Females Against Pulmonary Hypertension
Article Snippet: Negative selection was performed using EasySep TM Rat CD4 + T Cell Isolation Kit (#19642, STEMCELL Technologies) followed by labeling CD4 + T cells with anti-CD25-PE antibody; the CD25 high cells subset was selected with the EasySep TM PE Selection Kit (#18557, STEMCELL Technologies).

Article Title: Cardiac injection of USSC boosts remuscularization of the infarcted heart by shaping the T-cell response.
Article Snippet: Coculture of USSC with CD4+ T cells Rat CD4+ T cells were magnetically sorted by EasySep Rat CD4+ T Cell Isolation Kit (StemCell).

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model.
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4+ T cells were enriched using the EasySep Rat CD4+ T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Mesenchymal stem cells exert renoprotection via extracellular vesicle-mediated modulation of M2 macrophages and spleen-kidney network
Article Snippet: Magnetic cell separation was performed to harvest CD4-positive cell-rich samples using an EasySep rat CD4 + T-cell isolation kit (STEMCELL Technologies, Vancouver, BC, Canada).

Article Title: Leflunomide ameliorates experimental autoimmune myasthenia gravis by regulating humoral and cellular immune responses.
Article Snippet: Leflunomide, an immunosuppressive disease-modifying anti-rheumatic drug (DMARD), is widely used in the treatment of rheumatoid arthritis (RA), psoriatic arthritis (PA) as well as multiple sclerosis.. However, its role in myasthenia gravis (MG) has not yet been clearly explored.. Here, we investigated the effect of leflunomide on experimental autoimmune myasthenia gravis (EAMG) in vivo and in vitro.

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySep Rat CD4 + T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Targeting HIF-1α alleviates the inflammatory responses and rebuilds the CD4 + T cell subsets balance in the experimental autoimmune myasthenia gravis inflammation model via regulating cellular and humoral immunity.
Article Snippet: Background: Cells and tissues in an inflammatory state are usually hypoxic.. The hypoxic environment can affect the differentiation of immune cells and produce Hypoxia-inducible Factor-1α (HIF-1α).. Inflammation is also a major contributor to the development and deterioration of Myasthenia Gravis (MG).

Derivative Assay:

Article Title: Identification of essential modules regulating T cell migration to the central nervous system in multiple sclerosis
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySepTM Rat CD4 + T Cell Isolation Kit (StemCell technologies, 19642), before purification by sorting.

Article Title: A Dominant Role for Regulatory T Cells in Protecting Females Against Pulmonary Hypertension
Article Snippet: Negative selection was performed using EasySep TM Rat CD4 + T Cell Isolation Kit (#19642, STEMCELL Technologies) followed by labeling CD4 + T cells with anti-CD25-PE antibody; the CD25 high cells subset was selected with the EasySep TM PE Selection Kit (#18557, STEMCELL Technologies).

Article Title: Cardiac injection of USSC boosts remuscularization of the infarcted heart by shaping the T-cell response.
Article Snippet: Coculture of USSC with CD4+ T cells Rat CD4+ T cells were magnetically sorted by EasySep Rat CD4+ T Cell Isolation Kit (StemCell).

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model.
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4+ T cells were enriched using the EasySep Rat CD4+ T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Mesenchymal stem cells exert renoprotection via extracellular vesicle-mediated modulation of M2 macrophages and spleen-kidney network
Article Snippet: Magnetic cell separation was performed to harvest CD4-positive cell-rich samples using an EasySep rat CD4 + T-cell isolation kit (STEMCELL Technologies, Vancouver, BC, Canada).

Article Title: Leflunomide ameliorates experimental autoimmune myasthenia gravis by regulating humoral and cellular immune responses.
Article Snippet: Leflunomide, an immunosuppressive disease-modifying anti-rheumatic drug (DMARD), is widely used in the treatment of rheumatoid arthritis (RA), psoriatic arthritis (PA) as well as multiple sclerosis.. However, its role in myasthenia gravis (MG) has not yet been clearly explored.. Here, we investigated the effect of leflunomide on experimental autoimmune myasthenia gravis (EAMG) in vivo and in vitro.

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySep Rat CD4 + T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Targeting HIF-1α alleviates the inflammatory responses and rebuilds the CD4 + T cell subsets balance in the experimental autoimmune myasthenia gravis inflammation model via regulating cellular and humoral immunity.
Article Snippet: Background: Cells and tissues in an inflammatory state are usually hypoxic.. The hypoxic environment can affect the differentiation of immune cells and produce Hypoxia-inducible Factor-1α (HIF-1α).. Inflammation is also a major contributor to the development and deterioration of Myasthenia Gravis (MG).

Cell Culture:

Article Title: Identification of essential modules regulating T cell migration to the central nervous system in multiple sclerosis
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySepTM Rat CD4 + T Cell Isolation Kit (StemCell technologies, 19642), before purification by sorting.

Article Title: A Dominant Role for Regulatory T Cells in Protecting Females Against Pulmonary Hypertension
Article Snippet: Negative selection was performed using EasySep TM Rat CD4 + T Cell Isolation Kit (#19642, STEMCELL Technologies) followed by labeling CD4 + T cells with anti-CD25-PE antibody; the CD25 high cells subset was selected with the EasySep TM PE Selection Kit (#18557, STEMCELL Technologies).

Article Title: Cardiac injection of USSC boosts remuscularization of the infarcted heart by shaping the T-cell response.
Article Snippet: Coculture of USSC with CD4+ T cells Rat CD4+ T cells were magnetically sorted by EasySep Rat CD4+ T Cell Isolation Kit (StemCell).

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model.
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4+ T cells were enriched using the EasySep Rat CD4+ T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Mesenchymal stem cells exert renoprotection via extracellular vesicle-mediated modulation of M2 macrophages and spleen-kidney network
Article Snippet: Magnetic cell separation was performed to harvest CD4-positive cell-rich samples using an EasySep rat CD4 + T-cell isolation kit (STEMCELL Technologies, Vancouver, BC, Canada).

Article Title: Leflunomide ameliorates experimental autoimmune myasthenia gravis by regulating humoral and cellular immune responses.
Article Snippet: Leflunomide, an immunosuppressive disease-modifying anti-rheumatic drug (DMARD), is widely used in the treatment of rheumatoid arthritis (RA), psoriatic arthritis (PA) as well as multiple sclerosis.. However, its role in myasthenia gravis (MG) has not yet been clearly explored.. Here, we investigated the effect of leflunomide on experimental autoimmune myasthenia gravis (EAMG) in vivo and in vitro.

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySep Rat CD4 + T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Targeting HIF-1α alleviates the inflammatory responses and rebuilds the CD4 + T cell subsets balance in the experimental autoimmune myasthenia gravis inflammation model via regulating cellular and humoral immunity.
Article Snippet: Background: Cells and tissues in an inflammatory state are usually hypoxic.. The hypoxic environment can affect the differentiation of immune cells and produce Hypoxia-inducible Factor-1α (HIF-1α).. Inflammation is also a major contributor to the development and deterioration of Myasthenia Gravis (MG).

CRISPR:

Article Title: Identification of essential modules regulating T cell migration to the central nervous system in multiple sclerosis
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySepTM Rat CD4 + T Cell Isolation Kit (StemCell technologies, 19642), before purification by sorting.

Article Title: A Dominant Role for Regulatory T Cells in Protecting Females Against Pulmonary Hypertension
Article Snippet: Negative selection was performed using EasySep TM Rat CD4 + T Cell Isolation Kit (#19642, STEMCELL Technologies) followed by labeling CD4 + T cells with anti-CD25-PE antibody; the CD25 high cells subset was selected with the EasySep TM PE Selection Kit (#18557, STEMCELL Technologies).

Article Title: Cardiac injection of USSC boosts remuscularization of the infarcted heart by shaping the T-cell response.
Article Snippet: Coculture of USSC with CD4+ T cells Rat CD4+ T cells were magnetically sorted by EasySep Rat CD4+ T Cell Isolation Kit (StemCell).

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model.
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4+ T cells were enriched using the EasySep Rat CD4+ T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Mesenchymal stem cells exert renoprotection via extracellular vesicle-mediated modulation of M2 macrophages and spleen-kidney network
Article Snippet: Magnetic cell separation was performed to harvest CD4-positive cell-rich samples using an EasySep rat CD4 + T-cell isolation kit (STEMCELL Technologies, Vancouver, BC, Canada).

Article Title: Leflunomide ameliorates experimental autoimmune myasthenia gravis by regulating humoral and cellular immune responses.
Article Snippet: Leflunomide, an immunosuppressive disease-modifying anti-rheumatic drug (DMARD), is widely used in the treatment of rheumatoid arthritis (RA), psoriatic arthritis (PA) as well as multiple sclerosis.. However, its role in myasthenia gravis (MG) has not yet been clearly explored.. Here, we investigated the effect of leflunomide on experimental autoimmune myasthenia gravis (EAMG) in vivo and in vitro.

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySep Rat CD4 + T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Targeting HIF-1α alleviates the inflammatory responses and rebuilds the CD4 + T cell subsets balance in the experimental autoimmune myasthenia gravis inflammation model via regulating cellular and humoral immunity.
Article Snippet: Background: Cells and tissues in an inflammatory state are usually hypoxic.. The hypoxic environment can affect the differentiation of immune cells and produce Hypoxia-inducible Factor-1α (HIF-1α).. Inflammation is also a major contributor to the development and deterioration of Myasthenia Gravis (MG).

Generated:

Article Title: Identification of essential modules regulating T cell migration to the central nervous system in multiple sclerosis
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySepTM Rat CD4 + T Cell Isolation Kit (StemCell technologies, 19642), before purification by sorting.

Article Title: A Dominant Role for Regulatory T Cells in Protecting Females Against Pulmonary Hypertension
Article Snippet: Negative selection was performed using EasySep TM Rat CD4 + T Cell Isolation Kit (#19642, STEMCELL Technologies) followed by labeling CD4 + T cells with anti-CD25-PE antibody; the CD25 high cells subset was selected with the EasySep TM PE Selection Kit (#18557, STEMCELL Technologies).

Article Title: Cardiac injection of USSC boosts remuscularization of the infarcted heart by shaping the T-cell response.
Article Snippet: Coculture of USSC with CD4+ T cells Rat CD4+ T cells were magnetically sorted by EasySep Rat CD4+ T Cell Isolation Kit (StemCell).

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model.
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4+ T cells were enriched using the EasySep Rat CD4+ T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Mesenchymal stem cells exert renoprotection via extracellular vesicle-mediated modulation of M2 macrophages and spleen-kidney network
Article Snippet: Magnetic cell separation was performed to harvest CD4-positive cell-rich samples using an EasySep rat CD4 + T-cell isolation kit (STEMCELL Technologies, Vancouver, BC, Canada).

Article Title: Leflunomide ameliorates experimental autoimmune myasthenia gravis by regulating humoral and cellular immune responses.
Article Snippet: Leflunomide, an immunosuppressive disease-modifying anti-rheumatic drug (DMARD), is widely used in the treatment of rheumatoid arthritis (RA), psoriatic arthritis (PA) as well as multiple sclerosis.. However, its role in myasthenia gravis (MG) has not yet been clearly explored.. Here, we investigated the effect of leflunomide on experimental autoimmune myasthenia gravis (EAMG) in vivo and in vitro.

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySep Rat CD4 + T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Targeting HIF-1α alleviates the inflammatory responses and rebuilds the CD4 + T cell subsets balance in the experimental autoimmune myasthenia gravis inflammation model via regulating cellular and humoral immunity.
Article Snippet: Background: Cells and tissues in an inflammatory state are usually hypoxic.. The hypoxic environment can affect the differentiation of immune cells and produce Hypoxia-inducible Factor-1α (HIF-1α).. Inflammation is also a major contributor to the development and deterioration of Myasthenia Gravis (MG).

Staining:

Article Title: Identification of essential modules regulating T cell migration to the central nervous system in multiple sclerosis
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySepTM Rat CD4 + T Cell Isolation Kit (StemCell technologies, 19642), before purification by sorting.

Article Title: A Dominant Role for Regulatory T Cells in Protecting Females Against Pulmonary Hypertension
Article Snippet: Negative selection was performed using EasySep TM Rat CD4 + T Cell Isolation Kit (#19642, STEMCELL Technologies) followed by labeling CD4 + T cells with anti-CD25-PE antibody; the CD25 high cells subset was selected with the EasySep TM PE Selection Kit (#18557, STEMCELL Technologies).

Article Title: Cardiac injection of USSC boosts remuscularization of the infarcted heart by shaping the T-cell response.
Article Snippet: Coculture of USSC with CD4+ T cells Rat CD4+ T cells were magnetically sorted by EasySep Rat CD4+ T Cell Isolation Kit (StemCell).

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model.
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4+ T cells were enriched using the EasySep Rat CD4+ T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Mesenchymal stem cells exert renoprotection via extracellular vesicle-mediated modulation of M2 macrophages and spleen-kidney network
Article Snippet: Magnetic cell separation was performed to harvest CD4-positive cell-rich samples using an EasySep rat CD4 + T-cell isolation kit (STEMCELL Technologies, Vancouver, BC, Canada).

Article Title: Leflunomide ameliorates experimental autoimmune myasthenia gravis by regulating humoral and cellular immune responses.
Article Snippet: Leflunomide, an immunosuppressive disease-modifying anti-rheumatic drug (DMARD), is widely used in the treatment of rheumatoid arthritis (RA), psoriatic arthritis (PA) as well as multiple sclerosis.. However, its role in myasthenia gravis (MG) has not yet been clearly explored.. Here, we investigated the effect of leflunomide on experimental autoimmune myasthenia gravis (EAMG) in vivo and in vitro.

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySep Rat CD4 + T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Targeting HIF-1α alleviates the inflammatory responses and rebuilds the CD4 + T cell subsets balance in the experimental autoimmune myasthenia gravis inflammation model via regulating cellular and humoral immunity.
Article Snippet: Background: Cells and tissues in an inflammatory state are usually hypoxic.. The hypoxic environment can affect the differentiation of immune cells and produce Hypoxia-inducible Factor-1α (HIF-1α).. Inflammation is also a major contributor to the development and deterioration of Myasthenia Gravis (MG).

Expressing:

Article Title: Identification of essential modules regulating T cell migration to the central nervous system in multiple sclerosis
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySepTM Rat CD4 + T Cell Isolation Kit (StemCell technologies, 19642), before purification by sorting.

Article Title: A Dominant Role for Regulatory T Cells in Protecting Females Against Pulmonary Hypertension
Article Snippet: Negative selection was performed using EasySep TM Rat CD4 + T Cell Isolation Kit (#19642, STEMCELL Technologies) followed by labeling CD4 + T cells with anti-CD25-PE antibody; the CD25 high cells subset was selected with the EasySep TM PE Selection Kit (#18557, STEMCELL Technologies).

Article Title: Cardiac injection of USSC boosts remuscularization of the infarcted heart by shaping the T-cell response.
Article Snippet: Coculture of USSC with CD4+ T cells Rat CD4+ T cells were magnetically sorted by EasySep Rat CD4+ T Cell Isolation Kit (StemCell).

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model.
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4+ T cells were enriched using the EasySep Rat CD4+ T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Mesenchymal stem cells exert renoprotection via extracellular vesicle-mediated modulation of M2 macrophages and spleen-kidney network
Article Snippet: Magnetic cell separation was performed to harvest CD4-positive cell-rich samples using an EasySep rat CD4 + T-cell isolation kit (STEMCELL Technologies, Vancouver, BC, Canada).

Article Title: Leflunomide ameliorates experimental autoimmune myasthenia gravis by regulating humoral and cellular immune responses.
Article Snippet: Leflunomide, an immunosuppressive disease-modifying anti-rheumatic drug (DMARD), is widely used in the treatment of rheumatoid arthritis (RA), psoriatic arthritis (PA) as well as multiple sclerosis.. However, its role in myasthenia gravis (MG) has not yet been clearly explored.. Here, we investigated the effect of leflunomide on experimental autoimmune myasthenia gravis (EAMG) in vivo and in vitro.

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySep Rat CD4 + T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Targeting HIF-1α alleviates the inflammatory responses and rebuilds the CD4 + T cell subsets balance in the experimental autoimmune myasthenia gravis inflammation model via regulating cellular and humoral immunity.
Article Snippet: Background: Cells and tissues in an inflammatory state are usually hypoxic.. The hypoxic environment can affect the differentiation of immune cells and produce Hypoxia-inducible Factor-1α (HIF-1α).. Inflammation is also a major contributor to the development and deterioration of Myasthenia Gravis (MG).

Fluorescence:

Article Title: Identification of essential modules regulating T cell migration to the central nervous system in multiple sclerosis
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySepTM Rat CD4 + T Cell Isolation Kit (StemCell technologies, 19642), before purification by sorting.

Article Title: A Dominant Role for Regulatory T Cells in Protecting Females Against Pulmonary Hypertension
Article Snippet: Negative selection was performed using EasySep TM Rat CD4 + T Cell Isolation Kit (#19642, STEMCELL Technologies) followed by labeling CD4 + T cells with anti-CD25-PE antibody; the CD25 high cells subset was selected with the EasySep TM PE Selection Kit (#18557, STEMCELL Technologies).

Article Title: Cardiac injection of USSC boosts remuscularization of the infarcted heart by shaping the T-cell response.
Article Snippet: Coculture of USSC with CD4+ T cells Rat CD4+ T cells were magnetically sorted by EasySep Rat CD4+ T Cell Isolation Kit (StemCell).

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model.
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4+ T cells were enriched using the EasySep Rat CD4+ T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Mesenchymal stem cells exert renoprotection via extracellular vesicle-mediated modulation of M2 macrophages and spleen-kidney network
Article Snippet: Magnetic cell separation was performed to harvest CD4-positive cell-rich samples using an EasySep rat CD4 + T-cell isolation kit (STEMCELL Technologies, Vancouver, BC, Canada).

Article Title: Leflunomide ameliorates experimental autoimmune myasthenia gravis by regulating humoral and cellular immune responses.
Article Snippet: Leflunomide, an immunosuppressive disease-modifying anti-rheumatic drug (DMARD), is widely used in the treatment of rheumatoid arthritis (RA), psoriatic arthritis (PA) as well as multiple sclerosis.. However, its role in myasthenia gravis (MG) has not yet been clearly explored.. Here, we investigated the effect of leflunomide on experimental autoimmune myasthenia gravis (EAMG) in vivo and in vitro.

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySep Rat CD4 + T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Targeting HIF-1α alleviates the inflammatory responses and rebuilds the CD4 + T cell subsets balance in the experimental autoimmune myasthenia gravis inflammation model via regulating cellular and humoral immunity.
Article Snippet: Background: Cells and tissues in an inflammatory state are usually hypoxic.. The hypoxic environment can affect the differentiation of immune cells and produce Hypoxia-inducible Factor-1α (HIF-1α).. Inflammation is also a major contributor to the development and deterioration of Myasthenia Gravis (MG).

Standard Deviation:

Article Title: Identification of essential modules regulating T cell migration to the central nervous system in multiple sclerosis
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySepTM Rat CD4 + T Cell Isolation Kit (StemCell technologies, 19642), before purification by sorting.

Article Title: A Dominant Role for Regulatory T Cells in Protecting Females Against Pulmonary Hypertension
Article Snippet: Negative selection was performed using EasySep TM Rat CD4 + T Cell Isolation Kit (#19642, STEMCELL Technologies) followed by labeling CD4 + T cells with anti-CD25-PE antibody; the CD25 high cells subset was selected with the EasySep TM PE Selection Kit (#18557, STEMCELL Technologies).

Article Title: Cardiac injection of USSC boosts remuscularization of the infarcted heart by shaping the T-cell response.
Article Snippet: Coculture of USSC with CD4+ T cells Rat CD4+ T cells were magnetically sorted by EasySep Rat CD4+ T Cell Isolation Kit (StemCell).

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model.
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4+ T cells were enriched using the EasySep Rat CD4+ T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Mesenchymal stem cells exert renoprotection via extracellular vesicle-mediated modulation of M2 macrophages and spleen-kidney network
Article Snippet: Magnetic cell separation was performed to harvest CD4-positive cell-rich samples using an EasySep rat CD4 + T-cell isolation kit (STEMCELL Technologies, Vancouver, BC, Canada).

Article Title: Leflunomide ameliorates experimental autoimmune myasthenia gravis by regulating humoral and cellular immune responses.
Article Snippet: Leflunomide, an immunosuppressive disease-modifying anti-rheumatic drug (DMARD), is widely used in the treatment of rheumatoid arthritis (RA), psoriatic arthritis (PA) as well as multiple sclerosis.. However, its role in myasthenia gravis (MG) has not yet been clearly explored.. Here, we investigated the effect of leflunomide on experimental autoimmune myasthenia gravis (EAMG) in vivo and in vitro.

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySep Rat CD4 + T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Targeting HIF-1α alleviates the inflammatory responses and rebuilds the CD4 + T cell subsets balance in the experimental autoimmune myasthenia gravis inflammation model via regulating cellular and humoral immunity.
Article Snippet: Background: Cells and tissues in an inflammatory state are usually hypoxic.. The hypoxic environment can affect the differentiation of immune cells and produce Hypoxia-inducible Factor-1α (HIF-1α).. Inflammation is also a major contributor to the development and deterioration of Myasthenia Gravis (MG).

Labeling:

Article Title: Identification of essential modules regulating T cell migration to the central nervous system in multiple sclerosis
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySepTM Rat CD4 + T Cell Isolation Kit (StemCell technologies, 19642), before purification by sorting.

Article Title: A Dominant Role for Regulatory T Cells in Protecting Females Against Pulmonary Hypertension
Article Snippet: Negative selection was performed using EasySep TM Rat CD4 + T Cell Isolation Kit (#19642, STEMCELL Technologies) followed by labeling CD4 + T cells with anti-CD25-PE antibody; the CD25 high cells subset was selected with the EasySep TM PE Selection Kit (#18557, STEMCELL Technologies).

Article Title: Cardiac injection of USSC boosts remuscularization of the infarcted heart by shaping the T-cell response.
Article Snippet: Coculture of USSC with CD4+ T cells Rat CD4+ T cells were magnetically sorted by EasySep Rat CD4+ T Cell Isolation Kit (StemCell).

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model.
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4+ T cells were enriched using the EasySep Rat CD4+ T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Mesenchymal stem cells exert renoprotection via extracellular vesicle-mediated modulation of M2 macrophages and spleen-kidney network
Article Snippet: Magnetic cell separation was performed to harvest CD4-positive cell-rich samples using an EasySep rat CD4 + T-cell isolation kit (STEMCELL Technologies, Vancouver, BC, Canada).

Article Title: Leflunomide ameliorates experimental autoimmune myasthenia gravis by regulating humoral and cellular immune responses.
Article Snippet: Leflunomide, an immunosuppressive disease-modifying anti-rheumatic drug (DMARD), is widely used in the treatment of rheumatoid arthritis (RA), psoriatic arthritis (PA) as well as multiple sclerosis.. However, its role in myasthenia gravis (MG) has not yet been clearly explored.. Here, we investigated the effect of leflunomide on experimental autoimmune myasthenia gravis (EAMG) in vivo and in vitro.

Article Title: A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model
Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySep Rat CD4 + T Cell Isolation Kit (StemCell technologies), before purification by sorting.

Article Title: Targeting HIF-1α alleviates the inflammatory responses and rebuilds the CD4 + T cell subsets balance in the experimental autoimmune myasthenia gravis inflammation model via regulating cellular and humoral immunity.
Article Snippet: Background: Cells and tissues in an inflammatory state are usually hypoxic.. The hypoxic environment can affect the differentiation of immune cells and produce Hypoxia-inducible Factor-1α (HIF-1α).. Inflammation is also a major contributor to the development and deterioration of Myasthenia Gravis (MG).



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a , b T-distributed stochastic neighbor embedding (tSNE) implementation for flow cytometry data visualization of leukocyte subsets and T-cell subsets. The data are presented as color-coded leukocyte population overlays (left) or cell number density color-coded (right) in the tSNE space. a Kidney samples on day 3 were divided into the following cell clusters: CD45 + cells, T cells, B cells, neutrophils, and M1 and M2 macrophages. b Kidney samples on day 7 were divided into the following clusters: CD45 + cells, non-CD3 + cells, CD8 + T cells, <t>CD4</t> + T cells, and Tregs. c Frequency of renal leukocyte subsets in CD45 + cells analyzed by flow cytometry on days 3 and 7 (day 3, n = 5 per group; day 7, n = 8 per group). d The proportion of Ki67-positive cells in the renal Treg subset on day 3 ( n = 3 per group). All data are shown as means ± SD. * p ≤ 0.05, **p ≤ 0.01, *** p ≤ 0.001 as determined by ANOVA.
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Image Search Results


a , b T-distributed stochastic neighbor embedding (tSNE) implementation for flow cytometry data visualization of leukocyte subsets and T-cell subsets. The data are presented as color-coded leukocyte population overlays (left) or cell number density color-coded (right) in the tSNE space. a Kidney samples on day 3 were divided into the following cell clusters: CD45 + cells, T cells, B cells, neutrophils, and M1 and M2 macrophages. b Kidney samples on day 7 were divided into the following clusters: CD45 + cells, non-CD3 + cells, CD8 + T cells, CD4 + T cells, and Tregs. c Frequency of renal leukocyte subsets in CD45 + cells analyzed by flow cytometry on days 3 and 7 (day 3, n = 5 per group; day 7, n = 8 per group). d The proportion of Ki67-positive cells in the renal Treg subset on day 3 ( n = 3 per group). All data are shown as means ± SD. * p ≤ 0.05, **p ≤ 0.01, *** p ≤ 0.001 as determined by ANOVA.

Journal: Communications Biology

Article Title: Mesenchymal stem cells exert renoprotection via extracellular vesicle-mediated modulation of M2 macrophages and spleen-kidney network

doi: 10.1038/s42003-022-03712-2

Figure Lengend Snippet: a , b T-distributed stochastic neighbor embedding (tSNE) implementation for flow cytometry data visualization of leukocyte subsets and T-cell subsets. The data are presented as color-coded leukocyte population overlays (left) or cell number density color-coded (right) in the tSNE space. a Kidney samples on day 3 were divided into the following cell clusters: CD45 + cells, T cells, B cells, neutrophils, and M1 and M2 macrophages. b Kidney samples on day 7 were divided into the following clusters: CD45 + cells, non-CD3 + cells, CD8 + T cells, CD4 + T cells, and Tregs. c Frequency of renal leukocyte subsets in CD45 + cells analyzed by flow cytometry on days 3 and 7 (day 3, n = 5 per group; day 7, n = 8 per group). d The proportion of Ki67-positive cells in the renal Treg subset on day 3 ( n = 3 per group). All data are shown as means ± SD. * p ≤ 0.05, **p ≤ 0.01, *** p ≤ 0.001 as determined by ANOVA.

Article Snippet: Magnetic cell separation was performed to harvest CD4-positive cell-rich samples using an EasySep rat CD4 + T-cell isolation kit (STEMCELL Technologies, Vancouver, BC, Canada).

Techniques: Flow Cytometry

a The co-culture of whole bone marrow cells and ASCs. ASCs enhanced Treg induction in CD4 + T cells ( n = 5 per group). b The co-culture of CD4 + T cells sorted from bone marrow and ASCs ( n = 3 per group), c the co-culture of CD4 + T cells sorted from bone marrow and EVs derived from cultured ASCs ( n = 3 per group), and d the co-culture of CD4 + T cells sorted from bone marrow and M2 macrophages with EVs ( n = 4 per group) induced Tregs in CD4 + T cells. All data are shown as means ± SD. * p ≤ 0.05, ** p ≤ 0.01, and ** p ≤ 0.001 as determined by Welch’s t test.

Journal: Communications Biology

Article Title: Mesenchymal stem cells exert renoprotection via extracellular vesicle-mediated modulation of M2 macrophages and spleen-kidney network

doi: 10.1038/s42003-022-03712-2

Figure Lengend Snippet: a The co-culture of whole bone marrow cells and ASCs. ASCs enhanced Treg induction in CD4 + T cells ( n = 5 per group). b The co-culture of CD4 + T cells sorted from bone marrow and ASCs ( n = 3 per group), c the co-culture of CD4 + T cells sorted from bone marrow and EVs derived from cultured ASCs ( n = 3 per group), and d the co-culture of CD4 + T cells sorted from bone marrow and M2 macrophages with EVs ( n = 4 per group) induced Tregs in CD4 + T cells. All data are shown as means ± SD. * p ≤ 0.05, ** p ≤ 0.01, and ** p ≤ 0.001 as determined by Welch’s t test.

Article Snippet: Magnetic cell separation was performed to harvest CD4-positive cell-rich samples using an EasySep rat CD4 + T-cell isolation kit (STEMCELL Technologies, Vancouver, BC, Canada).

Techniques: Co-Culture Assay, Derivative Assay, Cell Culture

A , Scheme of the experimental design. Briefly, CD4 + T cells were collected from the plasma of healthy donors and CRISPR KOs were generated in vitro . The cells were treated with control medium, S1P as the endogenous S1PR1 agonist, or fingolimod the MS drug, and S1PR1 internalization was measured by FACS surface staining. B , Representative flow cytometry plots of control and KO T MBP cells for medium, S1P and fingolimod treatment conditions. C , Quantification of the S1PR1 internalization upon treatment. Values show the percentage of S1PR1 expressing cells (left) or the median fluorescence intensity (right) of the KO compared to the control (lilac line). A ratio of 1 indicates the surface S1PR1 expression levels of control T MBP cells, a ratio below 1 indicates reduced S1PR1 surface expression, a ratio above 1 increased S1PR1 surface expression. n = 1-3 independent internalization assays from 4 donors for all groups. C , One sample t-test against hypothetical mean = 1. Figures show mean ± s.d, P > 0.05 ns (non-significant), P < 0.05 *, P < 0.01 **, P < 0.001 ***, P < 0.0001 ****.

Journal: bioRxiv

Article Title: Identification of essential modules regulating T cell migration to the central nervous system in multiple sclerosis

doi: 10.1101/2022.06.17.496548

Figure Lengend Snippet: A , Scheme of the experimental design. Briefly, CD4 + T cells were collected from the plasma of healthy donors and CRISPR KOs were generated in vitro . The cells were treated with control medium, S1P as the endogenous S1PR1 agonist, or fingolimod the MS drug, and S1PR1 internalization was measured by FACS surface staining. B , Representative flow cytometry plots of control and KO T MBP cells for medium, S1P and fingolimod treatment conditions. C , Quantification of the S1PR1 internalization upon treatment. Values show the percentage of S1PR1 expressing cells (left) or the median fluorescence intensity (right) of the KO compared to the control (lilac line). A ratio of 1 indicates the surface S1PR1 expression levels of control T MBP cells, a ratio below 1 indicates reduced S1PR1 surface expression, a ratio above 1 increased S1PR1 surface expression. n = 1-3 independent internalization assays from 4 donors for all groups. C , One sample t-test against hypothetical mean = 1. Figures show mean ± s.d, P > 0.05 ns (non-significant), P < 0.05 *, P < 0.01 **, P < 0.001 ***, P < 0.0001 ****.

Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySep™ Rat CD4 + T Cell Isolation Kit (StemCell technologies, 19642), before purification by sorting.

Techniques: CRISPR, Generated, In Vitro, Staining, Flow Cytometry, Expressing, Fluorescence

A, scRNAseq experimental design. CD4 + T cells from the blood and CSF of untreated MS patients in relapse, or sex and age matched controls, were collected and analysed. B, CD4 + T cell clusters. C, Cluster-defining genes. D, Distribution of control or MS samples, and of blood or CSF samples, across the clusters. E, Overlapping cells in control, left, and MS, right, across clusters, defined as cells whose TCR was found in blood and CSF of the same patient. F, Correlations between the percentage of cells in the MS blood sample that have overlapping TCRs in the CSF, in a given cluster, and the relative gene expression level of essential regulators in this cluster in the blood of patients with MS. G, Violin plots of the candidate genes expression level across clusters, comparing control (lilac) and MS (green) cell samples from blood. Stars indicate significance as per adjusted p-value < 0.05 and absolute log2(Fold Change) > 3 times the standard deviation of the sample. H, Correlations between the percentage of cells in the MS sample that have overlapping TCRs in the CSF, in a given cluster, and the log2(Fold Change) between MS and control blood in this cluster, for those essential regulators that show differences in expression levels between MS compared to control blood as per G . E, H , R value and p-values correspond to a linear regression model.

Journal: bioRxiv

Article Title: Identification of essential modules regulating T cell migration to the central nervous system in multiple sclerosis

doi: 10.1101/2022.06.17.496548

Figure Lengend Snippet: A, scRNAseq experimental design. CD4 + T cells from the blood and CSF of untreated MS patients in relapse, or sex and age matched controls, were collected and analysed. B, CD4 + T cell clusters. C, Cluster-defining genes. D, Distribution of control or MS samples, and of blood or CSF samples, across the clusters. E, Overlapping cells in control, left, and MS, right, across clusters, defined as cells whose TCR was found in blood and CSF of the same patient. F, Correlations between the percentage of cells in the MS blood sample that have overlapping TCRs in the CSF, in a given cluster, and the relative gene expression level of essential regulators in this cluster in the blood of patients with MS. G, Violin plots of the candidate genes expression level across clusters, comparing control (lilac) and MS (green) cell samples from blood. Stars indicate significance as per adjusted p-value < 0.05 and absolute log2(Fold Change) > 3 times the standard deviation of the sample. H, Correlations between the percentage of cells in the MS sample that have overlapping TCRs in the CSF, in a given cluster, and the log2(Fold Change) between MS and control blood in this cluster, for those essential regulators that show differences in expression levels between MS compared to control blood as per G . E, H , R value and p-values correspond to a linear regression model.

Article Snippet: For spleen, erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4 + T cells were enriched using the EasySep™ Rat CD4 + T Cell Isolation Kit (StemCell technologies, 19642), before purification by sorting.

Techniques: Expressing, Standard Deviation